Biosafety of DNA vaccines: New generation of DNA vectors and current knowledge on the fate of plasmids after injection.

Florence Faurez (1) , Daniel Dory (1) , Vincent Le Moigne (1) , Rodolphe Gravier (1) , André Jestin (1)

1 Laboratoire d'études et de recherches avicoles, porcines et piscicoles

DNA vaccination has been widely studied to develop new, alternative, efficient and safe vaccines for humans and animals. Many efforts have been made to increase the immunising potential of these vaccines and three veterinary vaccines are now available on the market. Much work is also being dedicated to develop effective DNA vaccines for humans. However, this new vaccination technique raises issues concerning biosafety due to the nature of the vector, i.e. a DNA molecule that contains sequences of prokaryotic origin (e.g. genes for antibiotic resistance). This review describes the development of the new generation of DNA vectors that are partially or completely devoid of elements of prokaryotic origin and outlines the results of studies on the fate of plasmids after their injection in vivo.

Domaines: Vaccinologie

Florence Faurez, Daniel Dory, Vincent Le Moigne, Rodolphe Gravier, André Jestin. Biosafety of DNA vaccines: New generation of DNA vectors and current knowledge on the fate of plasmids after injection.. Vaccine, 2010, 28 (23), pp.3888-95. ⟨10.1016/j.vaccine.2010.03.040⟩. ⟨hal-00493412⟩