Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread.

Olivier Celle (1) , Philippe Blanchard (1) , Violaine Olivier (1) , Frank Schurr (1) , Nicolas Cougoule (1) , Jean-Paul Faucon (1) , Magali Ribière (1)

1 AFSSA - Agence Française de Sécurité Sanitaire des Aliments

Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral replication. These results suggest a new way by which the infection may be spread and other sites of viral persistence in the close apiary environment.

Mots clés: Chronic bee paralysis virus (CBPV); Camponotus vagus; Formica rufa; Varroa destructor; Quantitation; Minus-strand-specific RT-PCR; Apis mellifera

Domaines: Biologie moléculaire

Olivier Celle, Philippe Blanchard, Violaine Olivier, Frank Schurr, Nicolas Cougoule, et al.. Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread.. Virus Research, 2008, 133 (2), pp.280-4. ⟨10.1016/j.virusres.2007.12.011⟩. ⟨hal-00414769⟩